Broad bacterial screening can be performed using various singleplex or multiplex real-time PCR-based assays, detecting e.g., Haemophilus influenza, Moraxella catarrhalis, Mycoplasma pneumoniae, Legionella spec, Pseudomonas aeruginosa, Streptococcus pneumoniae, Staphylococcus aureus, and Streptococcus pyogenes.
16S ribosomal RNA (rRNA) gene sequencing is a common method based on broad spectrum amplication and sequencing of the 16srDNA which can be used to identify and compare bacteria present within a sample. The bacterial microbiome can be assessed by 16S-rDNA based next generation sequencing and quantification of the number of 16S-rDNA copies. This analysis provides a cross-sectional analysis of the bacterial genera and species present in a clinical samples. DDL has experience in the application microbiome analyses to study the impact of bacterial infections in various areas, including respiratory infections, various skin diseases, and bacterial vaginosis.
Besides microbiome analysis, DDL also offers whole genome sequence analysis of isolated bacterial strains. These whole genome data can be used for epidemiological purposes (e.g., MRSA outbreaks) or to support the clinical development of targeted anti-bacterial therapies. This service can be combined with traditional culture-based microbiology diagnostic services.